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Hey guess what... It fixed itself. Well maybe not completely by itself. In the preferences within Amadeus, I selected the onboard sound for the output, and then selected the USB audio interface again. That seemed to solve the problem. Not a very technical solution, but hey, after this many years muc...

Hi Jim Thanks for the reply. I'm using an Allen & Heath ZED Sixty 10FX mixer which is equipped with USB audio interface. It also happens when I use a Zoom H4n as audio interface. I'm using a mid 2015 Macbook Pro on El Capitan OSX 10.11.4 I've checked those three places, which I had already check...

Try using Audio Hijack. It's another Rogue Amoeba product. Records any sound your computer makes.

Just recently I notice that I get no playback through my USB audio interface. I can record into Amadeus, but when I play it back, the sound only comes through the onboard speakers of my Mac, and not through the USB audio interface. Yes, I've checked the option for input / output in preferences. I'm ...